| 个人简介 | |
|---|---|
 Prof. Jinping Zhang Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, China |
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| 标题： Integrated Analysis of Microbiota with Bile Acids for the Phototherapy Treatment of Neonatal Jaundice | |
| 摘要：
Objective: Infant jaundice is a common condition which results from a high concentration of serum bilirubin. Phototherapy is a widely-used treatment for bilirubin clearance.We analyzed the effect of phototherapy on intestinal flora and metabolism of newborns.
Methods: Fifty-three jaundiced infants hospitalized at our neonatal intensive care unit were treated with phototherapy. Of them, 29 were prescribed antibiotics during the hospitalization, and the others were not. Fecal samples were collected before and 24 h and 48 h after phototherapy. The bacterial species and relative abundance were identified with Macrogene sequencing. The bile acids in feces were identified using liquid chromatography-mass spectroscopy (LC-MS).
Results: Differential species and secondary bile acids were found after phototherapy. Among the infants who did not receive antibiotic treatment, the microbial species/genera Alistipes putredinis, Cellvibrio unclassified, Clostridium bolteae, Enterobacter cloacae, Enterococcus faecium, Eubacterium siraeum, Finegoldia magna, Veillonella atypica, Alistipes, Bacillus, Cellvibrio, Enterobacter, Finegoldia and Lactococcus were significantly altered. Secondary bile acids including LCA, 12-ketol LCA, β-UDCA, DCA, NorCA, GLCA, GUDCA, GDCA, TLCA, THDCA+TUDCA were also significantly altered. Among the infants who were given antibiotic treatment, the microbial species/genera Bacteroides caccae, Dorea unclassified, Eubacterium rectale, Faecalibacterium prausnitzii, Pantoea unclassified, Roseburia unclassified, Veillonella parvula, Veillonella unclassified, Clostridium, Eubacterium, Faecalibacterium, Pantoea, Prevotella, Roseburia and Veillonella were significantly altered. Secondary bile acids including LCA, isoLCA, NorCA, GLCA, GUDCA, GDCA, LCA-3S and TLCA were also significantly altered. The differential species/genera and the differential secondary bile acids interacted with each other.
Conclusions: This study identified several differential intestinal microbial species and secondary bile acids in fecal samples from infants with jaundice before and after phototherapy.Phototherapy can change the flora and its metabolism and Its long-term impact needs further observation.
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| 简介： Jinping Zhang MD，Ph.D Chief physician, Director of Pediatrics of Sixth People's Hospital Shanghai,postgraduate supervisor of Shanghai Jiaotong University Social service: Member of the premature infant group, the neonatology group of the Chinese Medical Association; Member of the pediatric neonatal branch of Shanghai Medical Association Main research: Neonatal Intestinal microflora Peer reviewer: Journal of Zhejiang University Medical Edition;Clinical pathology. Research projects: NSFC, education fund of the Ministry of education of China, and China Canada neonatal cooperation group. Publication: 30 papers, 15 SCI | |
